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Biochemistry miscellaneous e8cd6b5a

Rothera&;s test used for detection of

A
Proteins
B
Glucose
C
Fatty acid
D
Ketones
High-Yield Explanation
The presence of ketosis can be established by the detection of ketone bodies in urine by Rothera&;s test.Rothera&;s test: Saturate 5 mL of urine with solid ammonium sulfate. Add a few drops of freshly prepared sodium nitroprusside followed by 2 mL of liquor ammonia along the sides of the test tube. Development of a purple ring indicates the presence of ketone bodies in urine. Strip tests based on the same principle are also available.Ref: D M Vasudevan 7th edition Page no: 166

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