Which of the following is the best method for monitoring thiamine level in blood?
High-Yield Explanation
Thiamine deficiency is diagnosed by an increase in erythrocyte transketolase activity observed upon addition of thiamine pyrophosphate. The activation of apo-transketolase (the enzyme protein) in erythrocyte lysate by thiamine diphosphate added in vitro has become the accepted index of thiamine nutritional status. Ref: Harper's Illustrated Biochemistry, 26th Edition, Pages 488-489 ; Lippincott Biochemistry, 2nd Edition, Page 322 ; Textbook of Biochemistry By D M Vasudevan, 3rd Edition, Page 269