Which of the following does not undergo phosphorylation by protein kinases in Eukaryotes:

D i.e. AsparagineSerine, threonine and tyrosine undergo phosphorylation by protein kinase (in eukryotes) during post translational modifications- Extra carboxyl group is added to glutamate residue of blood clotting protein prothrombin (carboxylation). Lysine undergo methylation in some muscle proteins (mono & dimethyllysine), and calmodulin (trimethyl lysine); carboxy group of glutamate undergo methylation removing their negative charge.