Radioisotopes are used in the following technique except –

ELISA (Enzyme linked Immunoabsorbent Assay) is an enzyme-based immunoassay and does not use radioactive isotopes. A test similar to ELISA which uses radioactive isotope in place of the enzyme is known as Radioimmunoassay (RIA). Nucleic acid sequencing, mass spectroscopy & RIA are tested using radioisotopes.