Tsetse fly transmits -

Trypanosoma brucei It is the causative organism of sleeping sickness. T. brucei gambiense → West African sleeping sickness T. brucei rhodesiense → East African sleeping sickness Definitive host → Man Intermediate host → Tsetse fly Infective form to men → Metacyclic form (metacyclic trypomastigote) Habitat - The reticuloendothelial tissue of lymph node and spleen. - Blood - Connective tissue spaces of various organs. - C.S.F. - Intercellular spaces in the brain.