A 26 year old male with recent history of unprotected sexual intercourse comes to opd with complaints of painless, indurated ulcer over the glans which exuded clear serum. Bilateral painless inguinal lymphadenopathy is also present. The most appropriate diagnostic test is:
High-Yield Explanation
History of sexual intercourse, painless and hard ulcer (chancre) and painless inguinal lymphadenopathy is suggestive of primary syphilis.
Dark ground examination is done to demonstrate spirochetes.