Biochemistry General f3e23903 Which of the following is true for phosphofructokinase? A It uses Fructose 1,6 bisphosphate as substrate. B It generates ATP C It catalyses an irreversible reaction in glycolysis D It is activated by increased levels of ATP and citrate. High-Yield Explanation Phosphofructokinase catalyses the first committed step of glycolysis. It is the rate limiting enzyme of glycolysis. The reaction is irreversible.