Biochemistry General ece90f02 Number of ATP molecules and NADH formed in each cycle of glycolysis? A a) 4 ATP 2NADH B b) 2 ATP 2 NADH C c) 4 ATP 4 NADH D d) 2 ATP 4 NADH High-Yield Explanation - In glycoslysis 4 ATPs are produced in which 2 ATPs are utilized so a net gain of 2 ATPs 2 NADH molecules are produced.