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Biochemistry Transcription ec13c2b3

RNA polymerase has which activity -

A
Primase
B
Helicase
C
Ligase
D
Topoisomerase
High-Yield Explanation
Ans. is 'a' i.e., Primaseo DNA synthesis cannot commence with deoxyribonucleotides because DNA polymerase cannot add a mononucleotide to another mononucleotide.o Thus, DNA polymerase cannot initiate synthesis of complementary DNA synthesis strand of DNA on a totally single stranded template.o For this, they require RNA primer, which is a short piece of RNA formed by enzyme primase(RNA polymerase) using DNA as a template.o RNA primer is then extended by addition of deoxyribonucleotides.o Later on, the ribonucleotides of the primer are replaced by deoxyribonucleotides.# Note : Primase is actually a DNA primase which has RNA polymerase activity. This DNA primase is also called DNA polymerase a.

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