Urease test is positive for: March 2013

Ans. B i.e. Klebsiella Urease It catalyzes the hydrolysis of urea to produce ammonia and carbamate, the carbamate produced is subsequently degraded by spontaneous hydrolysis to produce another ammonia and carbonic acid. Urease activity tends to increase the pH of the environment in which it is as it produces ammonia, as it is a basic molecule. Ureases are found in numerous bacteria, fungi, algae, plants and some inveebrates, as well as in soils, as a soil enzyme. They are nickel-containing metalloenzymes of high molecular weight. Mnemonic (PUNCH-K): P-Proteus; U-Ureaplasma; N-Nocardia; C-Cryptococcus; H-Helicobacter; K: Klebsiella