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Biochemistry Basics of DNA ea220936

Nucleic acids absorb UV light maximally at the wavelength of

A
260nm
B
280 nm
C
410 nm
D
320 nm
High-Yield Explanation
Nucleic acids absorb UV light maximally at the wavelength of 260 nm 260/280 ratio of pure DNA/RNA is in the range of 1.8 to 2. Amino acids absorb U.V light (not visible light)- colorless. Aromatic amino acids absorb U.V light at 250-290 nm (maximum absorption is at 280nm) because of conjugated double bonds.

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