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Pathology Cellular Pathology e330356c

Principle of Prussian blue stain:

A
Ferrous to ferricyanide
B
Ferrocyanide to ferroferric cyanide
C
Ferroferriccyanide to ferrocyanide
D
Ferrocyanide to ferricferrocyanide
High-Yield Explanation
Ans. (b) Ferrocyanide to ferroferric cyanide(Ref: Dacie and Lewis Practical Haematology 11th ed, pg 338)Hemosiderin reacts with potassium ferrocyanide to form a blue compound, ferri ferrocyanide; this reaction is the basis of a positive Prussian-blue (Perls') reaction.

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