Northern blot technique is used in the detection of

Explanation: Southern Blotting: o Highly sensitive technique for identifying DNA fragments by DNA- DNA hybridization. o This technique has very wide applications in DNA analysis. Northern Blotting: o Used for the analysis of RNA. o Procedure is analogous to southern blotting, hence the name northern blotting. o Here the RNA mixture is separated by gel electrophoresis, blotted and identified using labelled DNA or RNA probes. Western Blotting: o A similar technique for the identification of proteins {antigens) is called immunoblotting {or, in conformity with other blotting techniques, western blotting). o Here the protein antigen mixture is separated by SDS-PAGE (sodium dodecylsulfatepolyacrylamide gel electrophoresis), blotted on to nitrocellulose strips and identified by radiolabeled or enzyme-labeled antibodies as probes. o E.g. Western blot test for detection of antibodies directed against different antigens of HIV.