True about Km

E + S --------------> E--S ---------------> E + PK2 If concentration of substrate is increased, the forward reaction K1 is increased, and so K3 as well as total velocity is correspondingly enhanced. The three different constants may be made into one equation,Km = K2 + K3 K1Km is called as Michaelis Constant.It is fuher shown thatVelocity (v) = Vmax Km + When concentration of substrate is made equal to Km, i.e.When = KmVelocity (v) = Vmax = Vmax = Vmax + 2 2or v = 1/2 VmaxRef: DM Vasudevan, Page no: 48