Which of the following procedures is routine technique for karyotyping using light microscopy:

G-banding which includes staining with Giemsa is the most common technique used for karyotyping Q-banding- fluorescent pattern obtained using quinacrine for staining C-banding- selective chromosome stain in which Giemsa stain is used to stain heterochromatic regions close to the centromeres V-staining- method for detecting apoptotic cells.