Gynaecology & Obstetrics General d8a7a3fd First histological sign of ovulation is A Vacuoles at apical portion of secretory nonciliated cells. B Cessation of glandular cell mitosis. C Absent glycoprotein in secretory non ciliated cells. D Subnuclear vacuoles and pseudostratification in basal portion of glandular epithelium. High-Yield Explanation After ovulation, glycogen accumulates in basal portion of glandular epithelium creating subnuclear vacuoles and pseudostratification.