Unwinding (denaturation) of dsDNA to provide as ssDNA template
B
Seals the single strand nick between the nascent chain and Okazaki fragments on lagging strand
C
Initiation of DNA synthesis and elongation
D
Initiates synthesis of RNA primers
High-Yield Explanation
DNA ligase Seals the single strand nick between the nascent chain and Okazaki fragments on lagging strandRef: Harpers Illustrated Biochemistry, 30th edition, page no: 382