Tertiary structure of protein is best determined by:

Ans. B. X- Ray diffraction crystallographyTertiary structure is best visualized by X-ray crystallography and NMR (Nuclear magnetic Resonance) spectroscopy. X-rayCrystallography was the first method used to determine the structure of proteins. X-ray crystallography is one of the best methods because the wavelength of an x-ray is similar to that of covalent bonds found throughout proteins, creating a clearer visualization of a molecule's structure. The scattering of x-rays by electrons is analyzed to determine the structure of proteins. In order to use x-ray crystallography, the protein in question must be in crystal form. Some proteins crystallize readily, while others do not. For those proteins that do not crystallize readily, nuclear magnetic resonance (NMR) spectroscopy must be used to determine its structure.