Anatomy General bd5df3f6 The middle cardiac vein is located in the: A Anterior interventricular sulcus. B Posterior interventricular sulcus. C Posterior AV groove. D Anterior AV groove. High-Yield Explanation The middle cardiac vein commences at the apex of the hea; ascends in the posterior longitudinal sulcus, and ends in the coronary sinus near its right extremity.