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Pathology Misc. (W.B.C) bca7c6ea

BCR-ABL fusion gene is detected by?

A
Flow cytometry
B
FISH
C
Karyotyping
D
RT-PCR
High-Yield Explanation
Ans. (b) FISH(Ref: Robbins 9th/pg 616-618)Flow cytometry: is used for immunophenotyping in Leukemias & LymphomasFISH- used for detecting submicroscopic chromosomal aberrations of BCR-ABL fusionKaryotyping: Used for detecting chromosomal defects of at least 5 mb size (low sensitivity)RT-PCR: used for quantitative DNA estimation

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