In sickle cell trait, number of bands found in Hb –

In hemoglobin electrophoresis, various types of hemoglobin move at varying speed. In sickle cell trait (heterozygous state), there are two types of hemoglobin - HbS (40%) and HbA (60%). So, on electrophoresis, two different bands are produced. In Homozygous state, almost all hemoglobin is HbS. So, a single band is produced. Laboratory findings of sickle cell anaemia Moderate to severe anaemia Peripheral smear will show Sickle cells                 Target cells A positive sickling test with a reducing substance like sodium metabisulfite. Howell - Jolly bodies because of autosplenectomy