A chromatographic technique which separates one protein from another based on the difference in their size is

Chromatographic techniques separate one protein from another based on the difference in their size (size exclusion chromatography), charge (ion-exchangechromatography), hydrophobicity (hydrophobic interaction chromatography), or ability to bind a specific ligand (affinity chromatography).Ref: Harper&;s Biochemistry; 30th edition; Chapter 4; Proteins: Determination of Primary Structure