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Microbiology Bacteria ae6256c5

Which one of the following factors released by heating a suspension of sheep erythrocytes is required for the growth of Haemophilus Influenzae in chocolate agar?

A
Hemin
B
Nicotinamide adenine dinucleotide (NAD)
C
Hemoglobin
D
Hemolysin
High-Yield Explanation
Ans. B. NAD.a. Members of Hemophilus require for one or more of the accessory factors: X & V in bloodb. Factor X is heme whereas factor V is NAD/NADP.c. The V factor (NAD) is trapped in the RBC's in blood agar. Hence, on blood agar, Hemophilus influenzae shows poor growth.d. By heating blood agar, V factor is released into the medium.

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