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Pathology General a6466fc3

Biopsy from an eight-year-old child with leg swelling showed smallround blue tumor cells consistent with diagnosis of Ewing's sarcoma. What will be the best method to detect translocation t(ll;22) in this malignancy?

A
Conventional karyotyping
B
Next generation sequencing
C
FISH
D
PCR
High-Yield Explanation
Ans. C. FISHFISH is a molecular cytogenetic technique that uses fluorescent probes that bind to only those pas of the chromosome with a high degree of sequence complementarity.

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