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Microbiology Bacteria a3e2c40f

The most sensitive method for detecting cervical Chlamydia trachomatis infection is:

A
Direct fluorescent antibody test
B
Enzyme immunoassay
C
Polymerase chain reaction
D
Culture on irradiated McConkey cells
High-Yield Explanation
Ans. is 'c' i.e., Polymerase chain reactionRef: Harrison, 17th/e, p. 1074,19th/e, p. 1165 and Ananthanarayan, 9th/e, p. 415Amplification assays such as ligase chain reaction and polymerase chain reaction are the most sensitive chlamydial diagnostic method.Other methods are:(a) Cell culture: Sensitivity (60-80%).(b) Direct immunofluorescent antibody test: Sensitivity 70-80% and quite specific.(c) ELISA: 60-80%.

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