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Biochemistry Techniques in molecular biology 9f251241

CRISPR/Cas9 mediated gene editing involves which of the following DNA repair mechanisms?

A
Base Excision Repair
B
Nucleotide Excision Repair
C
Non-Homologous End Joining
D
Mismatch Repair
High-Yield Explanation
CRISPR The CRISPR system represents a form of acquired immunity to infection by bacteriophages, which complements the system of restriction endonucleases and methylases. CRISPR uses RNA-based targeting to bring the Cas9 nuclease to foreign (or any complementary)DNA. Within bacteria this CRISPR-RNA-Cas9 complex degrades and inactivates targeted DNA. Use- gene deletion,gene editing and even modulation of gene transcription. CRISPR mediated gene therapy works on the basis of both homologous and non-homologous end joining DNA repair mechanism

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