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Microbiology Immunology 9e59ad74

Most sensitive test for antigen detection is -

A
RIA
B
Elisa
C
Immunofluorescence
D
Passive hemagglutination
High-Yield Explanation
RIA is a competitive binding assay in which fixed amounts of antibody and radiolabelled antigen react in the presence of unlabeled antigen. The labeled and unlabeled antigens compete for the limited binding sites on the antibody. This competition is determined by the level of the unlabelled antigen present in the patient's serum sample. Reference : Anathanarayan & paniker's 9th edition, pg no:113 <\p>

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