Biochemistry General 9e384746 Which of the following is a mitochondrial enzyme of Urea Cycle A Arginase B Carbamoyl Phosphate synthetase I C Arginosuccinate lyase D Arginosuccinate synthetase. High-Yield Explanation CPS - I is a mitochondrial enzyme and catalyses rate limiting step of Urea Cycle. All the Cytoplasmic enzymes of urea cycle start with letter "A".