Which of the following statement regarding banding is TRUE?

A band is defined as a pa of the chromosome that is clearly distinguishable from its adjacent segments by appearing darker or lighter with one or more banding technique. G banding is obtained with giemsa stain following digestion of chromosomes with trypsin. Trypsin treatment is frequently used during chromosome preparation for removal of cellular contaminants and fragment proteins. Treatment with trypsin causes chromosome swelling as a secondary effect due to a proteolytic action of this enzyme and induces chromosomal bands. Banding techniques are staining procedures which are available to produce a specific pattern of bands along the length of each chromosome. 3 types of banding technique are: Q banding G banding R banding Ref: Your Easy Way to Chromosomes By Sana Nimer Abu Shihab page 158