Oxidative decarboxylation of pyruvate requires

Inside the mitochondria, pyruvate is oxidatively decarboxylated to acetyl CoA by pyruvate dehydrogenase (PDH). It is a multi-enzyme complex with 5 co-enzymes and 3 apo-enzymes. The co-enzymes needed are: Thiamine pyrophosphate (TPP) Co-enzyme A (CoA) FAD NAD+ Lipoamide. The enzyme part of the PDH complex is made up of three component enzymes. Reference: Vasudevan 7th  ed, pg 116