Chlamydia are isolated by :

Isolation of chlamydiae can done by : Inoculationinto yolk sac/embryonated eggs of 6-8 day old chick embryo which may be pretreated by streptomycin or polymyxin Inculation into experimental animals (mice) Tissue/cell culture : Preferred mode Commonly used cell lines are McCoy and HeLa cells. Cell cultures are pretreated by irradiation or chemicals such as 5-iodo – 2 deoxyuridic or cycloheximide to enhance replication and detection of inclusion bodies. Pretreatment with DEAE dextrax or centrifugation after inoculation, promotes contact between chlamydiae particles and cell monolayer.