Dye which is NOT used in fluorescent microscopy:

Thioflavin is not used as a dye for fluorescent microscopy Fluorescent microscopy - Based on the principle that the specimens stained with fluorescent dye when exposed to ultraviolet light result in emission of longer wavelength of light (i.e. visible light). The bacteria stained with fluorescent dye appear as a brightly glowing object against a dark background *(F)-Fluorescent dye used commonly are -( FAL Rapid Fire) *(A)-Auramine *(L)-Lissamine *(Rapid)-Rhodamine *(Fire)-Fluoresceine isothiocynate =Congo red , Thioflavin T and Thioflavin S is used for staining in Amyloidosis =Brilliant Blue - used as a biological stain to stain cell walls, bacteria, and fungal cells.