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Biochemistry Basics of DNA 81b7311f

At Tm or melting temperature, what percentage of dsDNA is denatured?

A
25%
B
50%
C
75%
D
100%
High-Yield Explanation
Melting temperature or Tm Temperature when half (50%) of the helical structure is denatured. At lower temperature, the melted strands are re-associated; this is called annealing. When DNA is denatured, unstacking of bases occur resulting in increased absorbance at 260 nm-hyperchromicity

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