What is the staining technique used here
High-Yield Explanation
Acid-fast Pink bacilli in the blue background; M.tuberculosis Most isolates of Mycobacterium, Nocardia, and Rhodococcus species are acid-fast. Acid-fast bacteria are those that retain carbolfuchsin (basic fuchsin dissolved in a phenol-alcohol-water mixture) even when decolourized with hydrochloric acid in alcohol (due to mycolic acid in cell wall) Ref : Ananthanarayana textbook of Microbiology 9th edition Pgno : 13-14