Events of female reproductive cycle are best assessed by:-

Vaginal cytology can be done by scraping the lateral vaginal wall with a spatula and then looking at the cells on a stained slide. A Maturation Index (MI) is a ratio obtained through performing a random count of three major cell types (parabasal cells, intermediate cells and superficial cells) that are shed from the squamous epithelium. Parabasal cells are the least mature cells having not been affected by estrogen or progesterone. Intermediate cells display mild maturation, having been affected by progesterone, Superficial cells display the most maturity, having been affected by estrogen A predominance of Parabasal cells indicates an absence of estrogen stimulation, & that a predominance of superficial cells indicates estrogen stimulation. Vaginal cytology would be preferable to hormonal assay as end organ changes is the ultimate assessment of hormones in the body.