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Biochemistry Classification and metabolism of amino acids 7e00fb98

All of the following are true about the stationary phase used in high-pressure liquid chromatography, EXCEPT

A
Made up of fine paicles
B
Provides high resistance
C
Provides more surface area
D
Provides low resolution
High-Yield Explanation
Stationary phase in HPLC is made up of fine silicone paicles - option a is correct As there are a lot of fine paicles, the surface area is increased and resistance to the mobile phase is increased. That is why we apply high-pressure - option b and c are correct As the surface area is high, the chance interaction between stationary and mobile phase is high - good separation is achieved, i.e. resolution is high, not low - option d is wrong

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