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Biochemistry Techniques in molecular biology 7d314e15

What does the term nick translation refer to?

A
Translation of RNA into polypeptide
B
Regulation of gene expression
C
Technique to label DNA
D
Technique to digest DNA
High-Yield Explanation
A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond. Nick is created by DNAse I Nick translation is done by DNA polymerase I Nick sealing is done by DNA ligase Nick translation removes DNA ahead of a nick, synthesizes DNA behind nick and as a net result, moves the nick in the 5' to 3' direction. This propey is used to incorporate radio labeled nucleotides in DNA.

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