The total body radical burden can be estimated by which of the following methods?

The total body radical burden can be estimated by measuring the products of lipid peroxidation: FOX (Ferrous Oxidation in Xylenol) Assay  Lipid peroxides can be measured by the ferrous oxidation in xylenol orange (FOX) assay. Under acidic conditions, they oxidize Fe 2+ to Fe 3+, which forms a chromophore with xylenol orange. Estimation of Dialdehydes ‒ The dialdehydes formed from lipid peroxides can be measured by reaction with thiobarbituric acid, when they form a red fluorescent adduct. The results of this assay are generally reported as total thiobarbituric acid reactive substances,TBARS. Measurement of Pentane and Methane in Exhaled air ‒ Peroxidation of ω-6 polyunsaturated fatty acids leads to the formation of pentane, and of-ω3 polyunsaturated fatty acids to ethane, both of which can be measured in exhaled air. Reference- Review of biochemistry by Rebecca James Perumcheril Pg-412