Non-competitive enzyme inhibition leads to

The inhibitor combines with the enzymes by forming a covalent bond and then the reaction becomes irreversible. The velocity (Vmax) is reduced. But Km value is not changed, because the remaining enzyme molecules have the same affinity for the substrate. iv. Increasing the substrate concentration will abolish the competitive inhibition, but will not abolish non-competitive inhibition.Ref: DM Vasudevan, Page no: 53