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Biochemistry Metabolism of nucleic acids 7000d4c5

The following methods can be used to detect the point mutation in the beta (b)-globin gene that causes sickle cell anemia, except

A
Polymerase chain reaction with allele-specific oligonucleotide hybridization
B
Southern blot analysis
C
DNA sequencing
D
Nohern blot analysis
High-Yield Explanation
The nohern blot is a technique used in molecular biology research to study gene expression by detection of RNA(or isolated mRNA) in a sample.The technique has been used to show overexpression of oncogenes and downregulation of tumor-suppressor genes in cancerous cells when compared to &;normal&; tissue, as well as the gene expression in the rejection of transplanted organs.

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