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Biochemistry General 6fcf44c4

RNA primer in eukaryote is formed by ?

A
Ligase
B
RNA polymerase
C
Topoisomerase
D
Helicase
High-Yield Explanation
DNA synthesis cannot commence with deoxyribonucleotides because DNA polymerase cannot add a mononucleotide to another mononucleotide.Thus, DNA polymerase cannot initiate synthesis of complementary DNA synthesis strand of DNA on a totally single stranded template.For this, they require RNA primer, which is a sho piece of RNA formed by enzyme primase (RNA polymerase) using DNA as a template.RNA primer is then extended by addition of deoxyribonucleotides.Later on, the ribonucleotides of the primer are replaced by deoxyribonucleotides. Primase is actually a DNA primase which has RNA polymerase activity. This DNA primase is also called DNA polymerase.

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