In Anaerobic glycolysis, there is gain of -
High-Yield Explanation
Net gain in anaerobic glycolysis is only - 2 ATP. - 2 NADH are produced also (Glyceraldehyde-3-P dehydrogenase step) - But 2 NADH are used also (Lactate dehydrogenase step). Similarly - 2 NAD are produced also (Lactate dehydrogenase step) - But 2 NAD are used also (Glyceraldehyde-3-P dehydrogenase step). So there is no net gain of NADH or NAD.