Two same charged proteins can be separated by

Sephadex is used to separate low and high molecular weight molecules. Sephadex is a faster alternative to dialysis (de-salting), requiring a low dilution factor (as little as 1.4:1), with high activity recoveries. Sephadex is also used for buffer exchange and the removal of small molecules during the preparation of large biomolecules, such as ampholytes, detergents, radioactive or fluorescent labels, and phenol (during DNA purification).Ref: DM Vasudevan - Textbook of Biochemistry, 7th edition, page no: 451 fig no:35.8