Diagnosis of thalassaemia major is based on the detection of which of following hemoglobins?

The major red cell hemoglobin in thalassemia major is HbF (a2 g2), which is markedly elevated. Thalassemia minor/ trait is associated with increased levels of HbA2( a2 d2 ) HbA (a2 b2) accounts 95% to 98% of the total hemoglobin in normal adults. HbS is seen in sickle cell anemia. These hemoglobins can be detected by Hb high performance liquid chromatography (HPLC)/capillary electrophoresis.