Movement of protein from nucleus to cytoplasm can be seen by

B i.e. FRAPFRAP (fluorescence recovery after photobleaching) primarily measures rate of lateral diffusion of lipids and it can be used to investigate diffusion of biological macromolecules (such as proteins etc) including lateral diffusion in the plane of membrane and movements into & out of nucleus.- FRET is used to visualize biochemistry of living cells such as to measure cAMP and activity of PKA.AFM (atomic force microscopy) is used to yield 3D contour map of membrane (surfaces) and visualize membrane proteins (eg microdomains or rafts etc)