Net ATP formed in glycolysis is
High-Yield Explanation
ATP Formation in the Glycolysis pathway Reaction Catalyzed by Method of ATP Formation ATP per mol of Glucose Glyceraldehyde-3-phosphate dehydrogenase Respiratory chain oxidation of 2 NADH 5* Phosphoglycerate kinase Substrate-level phosphorylation 2 Pyruvate kinase Substrate-level phosphorylation 2 Total 9 Consumption of ATP for reactions of hexokinase and phosphofructokinase 2 Net 7 *This assumes that NADH formed in glycolysis is transpoed into mitochondria by the malate shuttle. If the glycerophosphate shuttle is used, then only 1.5 ATP will be formed per mol of NADH. Reference: Harper; 30th edition; Page no: 169