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Unknown General 6297f57d

Which one of the following about enzymes is true?

A
High Km-High affinity
B
Low Km-High affinity
C
Non-competitive inhibition- Decreases Km
D
Non-competitive inhibition – Increased Km
High-Yield Explanation
(Refer: DM Vasudevan, Textbook of Biochemistry, 7th edition, pg no: 59 - 60) The Michaelis constant (km) Km provides a measure of the affinity of the enzyme for its substrate. It is equal to substrate concentration at which reaction velocity is equal to ½ Vmax. Km = substrate concentration when reaction velocity is % Vmax. Km is constant for any given enzyme. Km is independent of substrate or enzyme concentration. The higher is KM, the higher is the concentration of the substrate required to reach ½ Vmax. That is: at a lower affinity, a higher concentration of substrate is required to reach ½  Vmax. ↓ Km = ↑ affinity.

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