True of malaria diagnosis -

1. Examination of peripheral blood smears (thin and thick blood films): Gold standard method: Thick smear: More sensitive (40 times), used for quantification and malaria pigment detection Thin smears: Used for speciation Feathery tail end of the smear should be examined At least 200-300 oil immersion fields should be examined before considered as negative. 2. Incubation periods- P. vivex: 14 days, P. falciparum: 12 days, P. malariae: 28 days, P. ovale: 17 days. 4. Ab detection in serum is not used for diagnosis of malaria. It is useful for - Epidemiological survey in malaria Screening of blood bank