Viruses can be isolated from clinical samples by cultivation in the following except -

Cultivation of viruses 'As viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium'. Three methods are employed for cultivation of viruses : 1. Animal Inoculation Earliest method Mice are most widely employed animals Other animals used are guinea pigs, rabbits and ferrets. 2. Embryonated eggs Sites of inoculation are : Chorioallantoic Membrane -----   visible pocks are produced → HSV, Vaccinia, Smallpox Amniotic Sac Allantoic Cavity  Influenza Some paramyxoviruses Yolk Sac Some viruses Chlamydia Rickettsia 3. Tissue culture Most important method for virus isolation. Three types of tissue culture are available Organ Culture Explant Culture Cell Culture - (routinely employed)