Indirect Coomb&;s test detects:

Indirect Coomb&;s test detects IgG antibodies in the serum (e.g. Anti-D Antibodies). Direct Coomb&;s test detects IgG Antibodies (or complements) attached to the surface of RBCs. Direct Antihuman Globulin Test Detects RBCs sensitized with IgG or Complements(C3B or C3d) (DAT; Coomb&;s; Direct Coomb&;s) Detects IgG Antibodies (or complements) attached to the surface of RBCs. Indirect Antihuman Globulin Test Direct Coomb&;s Test In the Direct Coomb&;s test, red blood cells (RBCs) sensitized with IgG antibodies (or C3b, C3d) are agglutinated when Coomb&;s reagent (Rabbit Anti-IgG antibody) is added to the test tube Detects Antibodies in the Serum Indirect Coomb&;s Test In the Indirect Coomb&;s test IgG antibodies (e.g. Anti-D) in the serum must first bind to blood group Type 0 Test RBCs added to the test tube. Addition of Coombs Reagent, then causes the sensitized Type 0 Test RBCs to agglutinate, indicating that IgG antibodies are present in the serum. REF : ananthanarayana 10th ed